毛竹竹青和竹黄半纤维素的提取与结构表征

闵斗勇; 黄曹兴; 李鑫; 何娟; 勇强

doi:10.3969/j.issn.0253-2417.2015.05.006

毛竹竹青和竹黄半纤维素的提取与结构表征

闵斗勇²,
黄曹兴¹,
李鑫¹,
何娟¹,
勇强^1, ,

1. 南京林业大学化学工程学院, 江苏南京 210037;
2. 南京林业大学轻工科学与工程学院, 江苏南京 210037

详细信息

通讯作者:
勇强,教授,博士生导师,主要从事生物质资源生物降解与转化的研究,E-mail:swhx@njfu.com.cn

中图分类号: TQ35
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出版历程
- 收稿日期: 2015-03-01
- 发布日期: 2015-10-27

Isolation and Characterization of Hemicellulose from Moso Bamboo Green and Bamboo Yellow

1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China;
2. College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, China

摘要

摘要: 以毛竹加工剩余物为原料,分离出竹青和竹黄。经粉碎过筛、苯/乙醇脱蜡和次氯酸钠脱木质素3个过程得到相应的综纤维素,然后用1%、5%和10%的KOH依次提取综纤维素得到半纤维素,竹青、竹黄半纤维素的总提取率分别为81.74%和85.36%。对所提取的半纤维素进行成分分析、分子量测定、红外光谱和核磁共振分析,结果表明,竹青、竹黄半纤维素成分主要为木糖,分别占61.02%~73.76%和65.22%~80.00%。竹黄半纤维素的重均分子质量为44 870~48 560 g/mol,高于竹青半纤维素的重均分子质量(43 970~46 245 g/mol)。竹青、竹黄碱溶半纤维素都是典型的阿拉伯糖基木聚糖结构,主链为β-D-吡喃木糖形成的木聚糖,在木糖基的C-2位连接着4-O-甲基-α-D-葡萄糖醛酸,C-3位连有α-L-呋喃阿拉伯糖,同时部分木质素通过苯苷键与半纤维素中的糖基相连。
- 竹青 /
- 竹黄 /
- 半纤维素 /
- 结构表征 /
- 核磁共振
Abstract: Bamboo green and bamboo yellow separated from Moso bamboo residues were delignified by NaClO2 after grinded, sifted and dewaxed by toluene-ethanol to achieve holocellulose. The resulted holocellulose was constantly extracted with 1%, 5% and 10% KOH to obtain hemicellulose. The yields of hemicellulose acquired from bamboo green and bamboo yellow were calculated as 81.74% and 85.36%, respectively. Then, the chemical compositions and structures of these hemicelluloses were characterized by HPAEC, GPC, FT-IR, 1H NMR and 2D HSQC techniques. The results indicated that the proportions of xylose in the bamboo green and bamboo yellow hemicellulose preparations were 61.02%-73.76% and 65.22%-80.00%, respectively. The weight average molecular weight of hemicellulose preparations of bamboo yellow were 44 870-48 560 g/mol, which were higher than that of bamboo green hemicellulose preparations (43 970-46 245 g/mol). It was demonstrated that the back chain of hemicelluloses were composed of β-D-xylan. Meanwhile, α-L-arabinofuranose and 4-O-methyl-α-D-glucuronic acid were linked to back chain of hemicellulose at C-3 and C-2 of xylan, respectively, and part of lignins were linked to hemicelluloses by phenyl glucoside bond.
- bamboo green /
- bamboo yellow /
- hemicellulose /
- characterization /
- NMR

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