红叶李叶化学成分及其体外抗氧化活性研究

纪小影; 尹浩; 卫强; 李前荣; 龙先顺

doi:10.3969/j.issn.0253-2417.2015.05.019

红叶李叶化学成分及其体外抗氧化活性研究

Chemical Constituents Extracted from Leaves of Prunus cerasifera Ehrh.cv. Atropurpurea Jacg. and Their Antioxidant Activities in vitro

摘要

摘要: 研究红叶李叶的化学成分和抗氧化活性。以硅胶、葡聚糖凝胶LH-20、高效制备液相色谱等分离红叶李叶乙醇提取物,根据光谱数据结合理化性质鉴定17个化合物,分别为β-谷甾醇( 1 )、豆甾醇( 2 )、胡萝卜苷( 3 )、羽扇豆醇 ( 4 )、熊果酸( 5 )、阿江榄仁酸( 6 )、Niga-ichigoside F1( 7 )、芹菜素 ( 8 )、山奈酚( 9 )、山柰酚-3-O-β-D-葡萄糖苷( 10 )、槲皮素( 11 )、槲皮素4'-O-β-D-葡萄糖苷( 12 )、金丝桃苷( 13 )、木犀草素( 14 )、木犀草素-4'-O-β-D-葡萄糖苷( 15 )、芦丁( 16 )、没食子酸( 17 )。所有化合物均为首次从红叶李叶中分离得到。以DPPH自由基清除能力检测所得化合物的抗氧化能力,结果显示,化合物 11 , 14 和 17 表现出显著的自由基清除能力,IC50分别为32.18、34.65和34.12 mmol/L,活性强于Vc(IC50为55.22 mmol/L)。

Abstract: Ethanol extracts from the leaves of Prunus cerasifera Ehrh.cv.Atropurpurea Jacg. were separated and purified by column chromatography over silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified by spectral methods including 1H, 13C NMR. Seventeen compounds were isolated and identified as β-sitosterol ( 1 ), stigmasterol ( 2 ), daucosterol ( 3 ), lupeol ( 4 ), ursolic acid ( 5 ), arjunolic acid ( 6 ), Niga-ichigoside F1 ( 7 ), apigenin ( 8 ), kaempferol ( 9 ), kaempferol-3-O-β-D-glucoside ( 10 ), quercetin ( 11 ), quercetin-4'-O-β-D- glucoside ( 12 ), hyperin ( 13 ), luteolin ( 14 ), luteolin-4'-O-β-D-glucoside ( 15 ), rutin ( 16 ), gallic acid ( 17 ). All compounds were isolated from the leaf for the first time. Compounds 11 , 14 and 17 showed stronger antioxidant activities by DPPH scavenging activity with IC50 inhibition rates of 32.18, 34.65 and 34.12 mmol/L, respectively, compared with the Vc IC50 inhibition rate of 55.22 mmol/L.

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