Abstract: Nanofiltration membrane was used to separate simulative biomass liquefaction oil solution consisting of glycerol and methyl glucosides(Meg). The effects of the parameters on NF400-600 spiral-wound nanofiltration membrane separation process were examined during the separation of glycerol and methyl glucosides. The optimum nanofiltration conditions established by single factor test were operating pressure 0.4 MPa, initial concentration 10 g/L, operating temperature 45 ℃. Under the above conditions, the permeate flux was 48.6 L/(m2·h), the retention ratio of methyl glucosides was 95.4%, and the penetration ratio of glycerol was 81.0%. Resistance model was used as the prototype to explore the mathematical models between membrane flux and operating parameters in the process of nanofiltration. The formula of J was J=0.625ΔP/(0.005 28+3.37×10-4ΔP0.017 7C0.901). The results of verification test showed the fitting effect of model agreed well with the average relative error < 6% and maximum relative error < 10%.