Abstract: Carbon supported ruthenium catalysts(Ru/CSB) were prepared with sugarcane bagasse as raw material by carbonization and impregnation method, which were used in the conversion of glucose to sorbitol. The structures of catalysts were characterized by XRD, SEM and TEM. The effects of reaction conditions on the performance and reusability of catalysts were investigated. Characterization results showed that Ru particles were well dispersed throughout the carbonized sugarcane bagasse(CSB). The glucose conversion of 99.41%, sorbitol yield of 98.13% and sorbitol selectivity of 98.71% could be obtained under the conditions of carbonization temperature of sugarcane bagasse 450℃, catalyst dosage(the mass percent of reaction system) 1%, H2 pressure 3 MPa, reaction temperature 120℃, reaction time 2 h. The reusabilities of catalysts were excellent. After using for five times, the dispersity of Ru declined, and the sorbitol yield slightly decreased to 94.80%.