Abstract: The liquefaction of bamboo biomass was investigated using ethanol as solvent with H2SO4 as catalyst in a 250 mL autoclave. The effects of different reaction conditions on the bamboo liquefaction were investigated. The results showed that optimum liquefaction conditions were catalyst dosage 5%, reaction temperature 180℃, reaction time 30 min. Under the circumstances, the yield of ethyl glycoside and phenolic products were 39.86% and 20.7%, respectively, with liquefaction ratio of 69.67%. The liquefied mixture was further separated by neutralization and step-wise separation process. Two platform chemicals, ethyl glycosides and phenolic products were obtained. The main compositions of phenolic products identified by GC-MS were 2,6-dimethoxyphenol, 3,5-dimethoxy-4-hydroxyphenylacetic acid, 4-allyl-2,6-dimethoxyphenol, and aspidinol et al, which accounting for 80% of the phenolic products. Five carbon sugars (furan glycosides) and six carbon sugars (glucopyranose and galactopyranoside) were the predominant products in glucosides, which accounting for 88.62% of the glycosides.