Abstract: The scale-up experiment of hot reflux extraction of total polyphenols, total flavonoids and oleuropein from olive leaves was studied. The effect of ethanol concentration on the extraction of active substances was investigated, and the related kinetics was also evaluated by ExpDec1 model. The results showed the highest contents of total polyphenols, total flavonoids and oleuropein were obtained when the ethanol concentration was 80% and they were 3.77, 2.85 and 3.03 g/L, respectively. ExpDec1 model had a good fitting effect, and the fitting degree R value was higher than 0.9. The higher ethanol concentration, the better the fitness effect observed. Under the condition of inlet temperature 150℃ and feeding rate 15 mL/min, the extracts were dried by spray drying, and crude extracts were purified by AB-8 macroporous resin, the content of total polyphenols increased from 9.71% to 31.65%, the content of total flavonoids increased from 7.22% to 10.98%, and the content of oleuropein increased from 9.16% to 20.38%. The physicochemical properties of the extract powder were measured, such as moisture content, porosity, density, dissolution, moisture absorption, active substance, etc. The results showed that moisture content, density and porosity of different extracts were similar, water extract had better water solubility(98.38%), 80% ethanol extract had higher content of the active substance(the contents of total polyphenols, total flavonoids and oleuropein were 9.71%, 7.22% and 9.16%, respectively), and the contents of total flavonoids and oleuropein were about two times higher than that of other solvents.