Abstract: The pine cones of Pinus pumila (Pall.)Regel were extracted by ethanol. From the resulted residues, the polysaccharides of P.pumila cones (PPCP) were obtained by hot water extraction, spray drying and alcohol precipitation processes. The content of the polysaccharides was determined by phenol sulfuric acid method. The monosaccharide composition analysis of the polysaccharide conducted by gas chromatography revealed that it was a heteropolysaccharide and consisted of L-arabinose, D-mannose, D-glucose and D-galactose in the molar ratio of 2.33:1.00:2.20:1.94, which indicated typical IR characteristics of polysaccharides. The antioxidant activity of polysaccharides was evaluated by DPPH free radical (DPPH·) scavenging capacity, ABTS free radical (·ABTS⁺) scavenging capacity and reducing capacity. The results showed that pine cones polysaccharides of P.pumila exhibited significant scavenging effects on DPPH·, ·ABTS⁺ and strong reducing power in a concentration-dependent manner. The maximum scavenging rate of polysaccharides against DPPH·and·ABTS⁺ were 39.63% and 39.63%, and the maximum reducing capacity was 0.78 at the concentration of 2.0 g/L. In addition, polysaccharides of P.pumila cones could stimulate RAW 264.7 macrophages to produce a large amount of NO, but had no effect on cell proliferation.