Abstract: Cellulose microfibril (CMF) was prepared from bleached softwood pulp by beating-high pressure homogenization process. The change of pulp fiber properties in beating process and the effects of homogenization process on the morphology and scale of CMF were studied. The results indicated that the beating process could effectively reduce the length of fiber and promote fiber microfibrization. The fibers with the Canadian standard freeness (CSF) of 0-800 mL and the length of 0.43-2.3 mm could be obtained by controlling the beating time 0-240 min. With the increase of homogenization passes, pressure and fiber mass concentration in homogenization process, the mechanical force strengthened, the microfibrization increased, the width of CMF decreased, and its width distribution tended to be concentrated. The multi-scale CMF with width from 406 nm to 134 nm could be prepared by controlling could the changes of homogenization passes (1-20 times), pressure (50-110 MPa) and fiber mass fraction(0.1%-0.5%). The beating-high pressure homogenization process could be used for environmentally friendly preparation of multi-scale CMF.