Abstract: The antifungal activities of petroleum ether extract(A), ethyl acetate extract(B) and n-butanol extract(C) of Rhizoma of Tupistra chinensis Baker against Penicillium digitatum, Aspergillus niger and Rhizopus stolonifer were investigated. The results showed that the antifungal activity of ethyl acetate extract was the strongest. On this basis, rosin resin(RAE) was used as coating agent, and the effects of preservatives(AR, BR and CR) with three kinds of Rhizoma extracts of T. chinensis(A, B and C) as antifungal agents on postharvest storage quality of ponkan were studied. The results showed that the petroleum ether extract and ethyl acetate extract in coating agent could effectively reduce the decay index of ponkan. After storage for 36 d, the decay indexes of ponkan treated by AR and BR coating were 10.6% and 7.2%, respectively, which were 6.1 and 9.5 percentage points lower than that of the blank control group(16.7%). At the same time, the addition of AR, BR and CR could delay the increase of malonaldehyde(MDA) content, the addition of AR and BR could delay the decrease of vitamin C and the addition of BR could keep the antioxidant enzyme activities at high level. However, AR, BR and CR had little effect on weight loss rate, hardness and soluble solids. Overall, the preservative BR had good preservative effect on postharvest ponkan.