Abstract: Corn straw enzymatic hydrolyzed lignin carbon LC₁, LC₂and LC₃ were prepared using purified corn straw enzymatic hydrolyzed lignin as carbon source and carbonized at high temperature under N₂ conditions for 1, 2 and 3 h. SEM, TEM and nitrogen adsorption/desorption curve were used to analyze the prepared carbon materials. The results showed that LC₁, LC₂ and LC₃ had microporous, mesoporous and macroporous structures with specific surface areas of 894.75, 1 376.74 and 776.47 m²/g, respectively. The pore volumes of the samples were 0.41, 0.70 and 0.40 cm³/g, respectively. XRD and Raman spectrum showed that the order of the enzymatic hydrolyzed lignin carbon increased with the prolongation of carbonization time. XPS analysis showed that the enzymatic hydrolyzed lignin carbon mainly contained C, O and N(small amount) elements. The electrochemical properties of LC₁, LC₂ and LC₃ were tested using a three-electrode system. The results showed that LC₂ had the highest specific capacitance of 222.2 F/g at 0.2 A/g current density, and the specific capacitance of LC₂ reached 149 F/g at higher current density of 20 A/g. Further study on its practical application in supercapacitors, the results showed that LC₂ assembled symmetric supercapacitor had the largest specific capacitance per electrode and the highest capacitance retention(70.2%). At 1 A/g current density, the LC₂ supercapacitor showed good rate performance and electrochemical stability after 5 000 charge-discharge cycles. Moreover, a series battery composed of symmetric LC₂ supercapacitor could directly light the LED lamp.