Abstract: A series of N-(α-eleostearic acyl) triazole compounds derived from α-eleostearic acid were synthesized: N-(α-eleostearic acyl)-3, 5-dibromo-1, 2, 4-triazole(3a), N-(α-eleostearic acyl)-1, 2, 4-triazole(3b), N-(α-eleostearic acyl)-3-sulfhydryl-1, 2, 4-triazole(3c), N-(α-eleostearic acyl)-5-nitro-1, 2, 4-triazole(3d), N-(α-eleostearic acyl)-1, 2, 3-benzotriazole(3e), 3-(α-eleostearic acyl)-1, 2, 4-triazole(3f), 4-(α-eleostearic acyl)-1, 2, 4-triazole(3g). The products were confirmed by FT-IR, ¹H NMR, ¹³C NMR and MS. The results of biological activity experiments showed that compound 3a and 3e had inhibitory effect on hepatocarcinoma cells Hep G2, rectal carcinoma cells DLD-1 and breast cancer cells MCF-7. The 50% inhibition concentration(IC₅₀) of compound 3d on MCF-7 was 25.12 μmol/L, which was better than the IC₅₀ of 5-fluorouracil(5-Fu). All compounds had good inhibitory activity against Candida albicans, and inhibitory activities on against Escherichia coli and Staphylococcus aureus were not significant. The IC₅₀ of compound 3e against Candida albicans was 22.96 mg/L, which was similar to 5-flucytosine and indicated that it had the potential of drug development.