Abstract: Focusing on alkali-treated bagasse, the effects of solid-liquid mixing methods, addition of three kinds of deep eutectic solvents(DES), feed method of fed-batch feeding, and the addition of xylanase on the enzymatic hydrolysis efficiency at high solid loading were investigated. The results showed that magnetic stirring could accelerate enzymatic hydrolysis speed, but had little effect on the efficiency of enzymatic hydrolysis. Negligible effect on enzymatic hydrolysis was observed by addition of low-concentration DES. A fed-batch with initial substrate loading of 150 g/L was fed at 12 and 36 h, respectively, which resulted in a total solid loading of 350 g/L for enzymatic hydrolysis. After enzymatic hydrolysis for 96 h, the total sugar concentration reached 162.7 g/L with the conversion rates of approximately 51% of both cellulose and hemicellulose. On the basis of fed-batch feeding, 1 000 IU/g hemicellulase was added, and the enzyme cocktail was used for enzymatic hydrolysis for 96 h. The total sugar concentration reached 218.9 g/L, and the conversion rate of cellulose and hemicellulose were 72% and 63%, respectively, which were 40% and 22% higher than those without the addition of hemicellulase.