Abstract: The methanol extract of the pink flowers of Bauhinia variegata L. was isolated and purified by chromatography on Sephadex LH-20, MCI gel CHP 20P and Toyopearl Butyl-650C were used to isolate and purify, a total of 14 compounds were obtained. The structures of the compounds were identified by NMR spectroscopy, and their tyrosinase inhibitory activities were screened by the method of tyrosinase-catalyzed levodopa oxidation rate. Fourteen compounds isolated and purified from the pink flowers of B. variegata, were identified as pyrocatechol(1), protocatechuic acid(2), phydroybenzoic acid(3), 2, 5-dihydroxy methyl phenylacetate(4), trans-p-coumaric acid(5), cis-p-coumaric acid(6), caffeic acid(7), ethyl caffeic acid(8), trans ferulic acid(9), guaiacol β-D-glucopyranoside(10), p-hydroxybenzoic acid glucose ester(11), (E)-2, 3-dihydroxycyclopentyl-3-(3′, 4′-dihydroxyphenyl) acrylate(12), chlorogenic acid(13), and isoorientin(14) by spectral analysis and comparison with literature data. Compounds 5, 6, 9, 10, 11, 12, 14 were obtained from this plant for the first time. The results of tyrosinase inhibition activity test showed that, compounds 2 and 7 showed relatively good tyrosinase inhibitory activity with IC₅₀ values of (4.804±0.939) g/L and (7.339±0.549) g/L, respectively. Their inhibitory activity increased with the increase of compound concentration, showing a positive dose-effect relationship.