Abstract: Molecular imprinted nanospheres(MINs) with high selective adsorption to 10-deacetyl baccatin Ⅲ(10-DAB) was prepared by 10-DAB as template molecule, and 4-vinylpyridine(4-VP) was used as functional monomer. The maximum adsorption capacity reached to 37.42 mg/g. The polymer was characterized by scanning electron microscope(SEM), Fourier transform infrared spectroscopy(FT-IR) and thermogravimetric(TG) analysis, which indicated that it was successfully synthesized. The adsorption kinetics of 10-DAB-MINs could be well described by the pseudo-second-order model, and R² was 0.979 5. Meanwhile, the adsorption mechanism conformed to the Langmuir-Freundlich model, and R² was 0.982 7. The cyclic adsorption results showed that 10-DAB-MINs had better recycling ability than that of the template free molecular imprinted nanaspheres(NINs). Furthermore, the adsorption capacity of 10-DAB-MINs could still reach 28.66 mg/g after recycling for 5 times, which indicated the good stability.