Abstract: In order to probe the high-value utilization of tobacco waste, the basic physicochemical properties of the feedstock as well as their thermal conversion characteristics were tested using TG, and Py-GC/MS, etc. Then, the pyrolysis experiment of tobacco waste was carried out in a fluidized bed reactor with a feeding rate of 10 kg/h. The results showed that tobacco waste had a very low bulk density and high porosity. The mass loss during tobacco pyrolysis was mainly occurred in the temperature range of 150-400 ℃, and the maximum rate of weight loss occurred near 180 and 315 ℃. The Py-GC/MS analysis of tobacco waste suggested that the bio-oil was mainly composed of nicotine, olefins, ketones, acids, aldehydes, alcohols, aromatics, phenols, pyridines and pyrroles. According to the pyrolysis experiment in the fluidized bed reactor, with the increase of pyrolysis temperature from 300 to 500 ℃, the yield of bio-oil reached its maximum of 32.58% at 450 ℃. The dominant compound in bio-oil was nicotine, followed by acids, ketones, phenols, and N-heterocyclic compounds. In addition, the gas yield increased gradually with the increasing temperature, which was mainly composed of CO₂ and CO, while the yield of solid char residue decreased.