Abstract: Using the roles of deep eutectic solvents(DES) in the molecularly imprinted polymers(MIP) as the main line, the roles of different components of DES in the preparation process of MIP were summarized. DES was used as the template molecule to enhance its water solubility, and make the synthesized MIP with good selectivity. DES could be used as the functional monomer or auxiliary functional monomer to increase the binding site with template molecules and effectively improve the affinity of the target. As the pore-forming agent, DES could reduce environmental pollution and improve pore permeability. DES was used as eluent to realize the advantages of environmental friendliness and low cost separation. DES could also modify the support of imprinted molecules on the surface, so that the required functional groups could be obtained after the support surface was loaded with DES, which was conducive to the preparation of molecule-imprinted polymers and could improve the adsorption performance of molecule-imprinted polymers. Meanwhile, a theoretical basis and development direction for the application of DES in the future green separation of molecular imprinting technology(MIT) was prospected.