Abstract: An HPLC method was developed for the simultaneous analysis of quinic acid(QA) and gallic acid(GA) in tara powder enzymatic digestion waste solution, and the methodological verification was carried out. The HPLC fingerprints were further drawn and the different batches of tara powder enzymatic digestion waste solution were evaluated by combining similarity and cluster analysis. The results showed that the detection wavelength at 215 nm, mobile phase acetonitrile-0.1% aqueous trifluoroacetic acid aqueous solution, acetonitrile gradient of 5%-20%, flow rate of 1.0 mL/min and column temperature of 30 ℃ were selected as the chromatographic analysis conditions for quinic acid and gallic acid. The linear range of quinic acid standard curve was 1.25-20 g/L, and the detection limit was 0.5 g/L. The linear range of gallic acid standard curve was 0.062 5-1 g/L, and the detection limit was 5 mg/L. The RSD of precision, repeatability, stability, and the spiked recovery test were less than 5%.The average spiked recoveries of quinic acid and gallic acid were 100.87% and 99.65%, respectively. The similarity of the fingerprint profiles of the 12 batches of waste samples was greater than 0.95. The samples could be divided into two categories by cluster analysis, with samples No.10 and No.11 were clustered into one category, and the rest samples were clustered into one category. The mass concentrations of quinic acid and gallic acid were calculated to be 482.3 g/L and 35.7 g/L, respectively.