Abstract: The extracts of Qinan(QN) and traditional agarwood(TA) were prepared by cold extraction(CE) and hot extraction(HE) with water and ethanol as solvent, respectively. The chemical constituents of the extracts were analyzed by GC-MS and HPLC, and their antioxidant and antibacterial activities were studied. The results showed that the species of sesquiterpenoids in QN were less than those of TA, while the contents of sesquiterpenoids in both were higher than that of TA. There was almost no tetrahydro-2-(2-phenylethyl) chromones(THPECs) in QN, and Flidersia type 2-(2-phenylethyl) chromones(FTPECs) was contained, where the mass fraction of 2-(2-phenethyl) chromone could reach 10.87%, and the mass fraction of 2-2-(4-methoxy)phenethylchromone was 4.80%. The species and contents of THPECs in TA were rich, and the mass fraction of agartetrol could reach 1.50%. With the increasing ethanol volume fraction(0-55%), the ABTS free radical scavenging ability and iron ion reduction ability of QN and TA extracts increased. Although the antioxidant activity of QN water extracts was lower than that of TA, while its ethanol extracts were higher than that of TA. QN and TA had no significant antibacterial activity against Escherichia coli, but their water extracts had antibacterial activity against Candida albicans.