Abstract: The curcumin/polylactic acid/glycolic acid(PLGA) magnetic microsphere was innovatively prepared by a stirring-homogenization two-step emulsification method using PLGA copolymer as drug loading material and oleic acid-modified triiron tetraoxide(Fe₃O₄@OA) nanoparticles as the magnetic functional material. Impacts of the emulsification method, high-speed homogenization speed, water oil volume ratio, PLGA dosage, mass fraction of aqueous polyvinyl alcohol(PVA), the dosage of Fe₃O₄@OA and curcumin on the particle size of magnetic microspheres were examined. The morphology and physicochemical properties of curcumin/PLGA magnetic microspheres were analyzed by optical microscope, laser particle sizer, ultraviolet spectrophotometer, vibrating sample magnetometer and thermogravimetric analyzer, respectively. The results showed that the optimal preparation process for curcumin/PLGA magnetic microspheres was the stirring homogenization two-step emulsification method, with a high-speed homogenization speed of 15 000 r/min, a water oil phase volume ratio of 10∶1, a PLGA dosage of 100 mg, a water phase PVA mass fraction of 1%, the Fe₃O₄@OA dosage of 40 mg and the dosage of 10 mg curcumin. Under these conditions, the surface of the microspheres was smooth, the particle size was homogeneous, the polydispersity index(PDI) was 0.38, the average particle size was 3.60 μm, the magnetic induction strength of the microspheres was 14.12 (A·m²)/kg, and the magnetic content was about 27.98%. The encapsulation rate of drug-laden microspheres was 97.09%, and the drug loading was 6.40%, which showed a significant sustained release effect in the in vitro release assay.