Abstract: This study designed and constructed an enzyme/magnetic dual-driven pectin(PET)/sodium alginate(SA) composite microsphere robot drug delivery system. Specifically, through the dropwise method, calcium lactate was used as a crosslinking agent to simultaneously load Fe₃KG-*6O₄ magnetic nanoparticles(Mag), catalase(CAT)/glucose oxidase(GOD)/zinc phosphate hybrid particles, and a natural camptothecin derivative(irinotecan hydrochloride CPT-11) drug into PET/SA composite microspheres, by which fabricated the magnetically responsive PET/SA composite microspheres(CPT-11/CAT/GOD-Zn₃(PO₄)₂@Mag/PET-SA). The effects of different compounding ratios of PET to SA and the addition of magnetic particles on the structure, morphology, particle size, and drug encapsulation efficiency of the composite microspheres were investigated. The drug release curves of the drug-loaded microsphere robots in simulated gastric fluid(SGF, pH value 1.2) and simulated intestinal fluid(SIF, pH value 6.8) were determined by ultraviolet spectrometry method. The results showed that the prepared enzyme/magnetic dual-driven microsphere drug-loading robot had good sphericity, but the addition of magnetic particles caused the appearance of a lot of wrinkles and become rough on the surface of the composite microspheres, leading to a significant decrease in drug encapsulation rate. When the ratio of m(PET) to m(SA) was 1∶3, the average particle size of the composite microspheres before the addition of magnetic particles was 1.658 mm, with an encapsulation rate of 63.1%. After the addition of magnetic particles, the average particle size of the composite microspheres increased up to 1.885 mm, and the encapsulation rate decreased up to 47.7%. The release of irinotecan hydrochloride drug was relatively slow in SGF while it released rapidly in SIF. Vibrating sample magnetometer(VSM) tests indicated that the composite microspheres exhibited superparamagnetism and high magnetization intensity, and could undergo self-driven in a glucose solution.