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龙竹竹叶不同极性溶剂萃取物的抗氧化及体外降血糖和降血脂活性研究

Antioxidant, Hypoglycemic and Hypolipidemic Activities in vitro of Different Polar Solvent Extracts from Dendrocalamus giganteus Leaves

  • 摘要: 以龙竹竹叶为原料,用体积分数70%乙醇进行提取后,采用石油醚、二氯甲烷、乙酸乙酯和正丁醇进行分级萃取,分别得到石油醚萃取物(PEE)、二氯甲烷萃取物(DME)、乙酸乙酯萃取物(EAE)、正丁醇萃取物(BE)和水提物(WE)等5种不同极性溶剂萃取物,测定了不同极性溶剂萃取物中总黄酮和多酚含量,并研究了各萃取物的抗氧化、体外降血糖和降血脂活性。结果表明:乙酸乙酯萃取物中的总黄酮和多酚质量分数均为最高,分别为(195.14±0.46)和(52.01±0.39) mg/g,显著高于其他萃取物(p<0.05)。不同极性溶剂萃取物均具有一定的抗氧化活性及体外降血糖和降血脂能力,其中乙酸乙酯萃取物清除ABTS+·、抑制α-葡萄糖苷酶和α-淀粉酶活性均为最强,半数抑制浓度(IC50值)分别为(118.73±0.29)、(45.74±3.31)和(53.81±2.59) mg/L;在相同质量浓度下,乙酸乙酯萃取物对甘氨胆酸钠和牛磺胆酸钠的结合率均为最高,质量浓度为0.25 g/L时,结合率分别达到(64.06±0.18)%和(59.27±0.35)%。相关性分析显示,龙竹竹叶不同萃取物中多酚含量与DPPH·和ABTS+·清除能力之间呈现出显著的正相关性(p<0.05),相关系数分别为0.94和0.81;总黄酮含量与体外降血糖和降血脂能力之间表现出显著的正相关性(p<0.05),相关系数为0.75~0.84。

     

    Abstract: The 70% ethanol extract from Dedrocalamus giganteus leaves was fractionated by successive extraction with petroleum ether, dichloromethane, ethyl acetate and n-butanol by graded extraction to obtain five different polar solvent extracts, including petroleum ether extract(PEE), dichloromethane extract(DME), ethyl acetate extract(EAE), n-butanol extract(BE), and water extract(WE), respectively. The contents of total flavonoids and polyphenols were determined, and the antioxidant, hypoglycemic and hypolipidemic activities of the five extracts in vitro were also evaluated. The results showed that the total flavonoid and polyphenols mass fractions of the ethyl acetate extract were the highest with(195.14±0.46) and(52.01±0.39) mg/g, respectively which were significantly higher than those of the other extracts(p < 0.05). Furthermore, all the different polar solvent extracts showed a certain antioxidant activity, hypoglycemic and hypolipidemic abilities in vitro. Among them, the ethyl acetate extract had the strongest scavenging activities on ABTS+ ·, inhibiting activities on α-glucosidase and α-amylase, and the half inhibitory concentration(IC50) values were(118.73±0.29), (45.74±3.31) and (53.81±2.59) mg/L, respectively. Meanwhile, the binding rates of the ethyl acetate extract with sodium glycinate and sodium taurocholate were the highest at the same concentration, which reached (64.06±0.18)% and (59.27±0.35)%, at a mass concentration of 0.25 g/L, respectively. Polyphenol content of different extracts of D. giganteus leaves showed significant positive correlation with DPPH · and ABTS+ · scavenging abilities(p < 0.05), with correlation coefficients of 0.94 and 0.81, respectively. The content of total flavonoids showed significant positive correlation with hypoglycemic and hypolipidemic ability in vitro (p < 0.05), with correlation coefficients were 0.75-0.84.

     

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