Abstract: Chemical constituents in the leaves of Sabina vulgaris Antoine were studied. Nine flavonoids were isolated and purified by extraction and different kinds of column chromatography, and their structures were elucidated as cupressuflavone(Ⅰ), cupressuflavone 4',4'''-dimethyl ether(Ⅱ), amentoflavone(Ⅲ), podocarpusflavone A(Ⅳ), catechin(Ⅴ), quercetin(Ⅵ), isoscutellarein 7-O-β-D-xylopyranoside (Ⅶ), myricetin 3-O-β-D-glucoside (Ⅷ), rutin(Ⅸ). Compounds Ⅰ, Ⅱ, Ⅴ, Ⅶ, Ⅷ and Ⅸ were obtained from this plant for the first time. Compounds Ⅷ and Ⅸ were isolated from Sabina genus for the first time. All of isolated compounds were tested for their antioxidative activities by 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH·) scavenging assay. It was found that compounds Ⅴ and Ⅵ showed antioxidative activity close to that of positive control, L-ascorbic acid, and the flavonoids glycosides (Ⅶ, Ⅷ and Ⅸ)are more active than the biflavones (Ⅰ, Ⅱ, Ⅲ and Ⅳ).