Abstract: Using seed kernel of yellow-horn (Xanthoceras sorbifolia Bunge) as raw material,extraction technique of conjugated linoleic acid (CLA)from X. sorbifolia was researched in the experiment, and CLA was analyzed and identified by UV-spectrophotometry. The results showed that the optimum conditions were identified as follows: ratio of propanediol:KOH 0.6:1(mol:L), reaction temperature 180℃, reaction time 2 h,yield 58.34%;the antioxidation activity of CLA could be obviously increased by addition of 0.01% tea polyphenols +0.07% span 40+0.02% citric acid. Peroxide number of CLA was affected by various environmental factors, among which temperature ranked the first, light intensity took the second place, and humidity was the last.