油茶叶乙醇提取物清除DPPH自由基作用的研究

Research on the Scavenging Effect of Ethanol Extracts from Camellia oleifera Abel Leaves on 2,2-Diphenyl-1-picrylhydrazyl Radical

摘要: 用二苯基苦基肼自由基(DPPH·)法测定了油茶叶乙醇提取物的抗氧化活性,并与芦丁、二丁基羟基甲苯(BHT)进行了比较,在波长17nm、反应时间40min的条件下测定了DPPH·的清除率为50%时抗氧化剂的质量浓度(EC50)值.结果表明,油茶叶乙醇提取物时DPPH·具有明显的清除作用,且随着油茶叶乙醇提取物纯化程度的提高,其清除能力也相应增强.测得油茶叶粗提物、油茶叶精提物、芦丁和BHT清除DPPH·的EC50值分别为12.19、6.765、9.481和37.53mg/L,清除DPPH·能力的大小顺序依次为油茶叶精提物>芦丁>油茶叶粗提物>BHT.

Abstract: Antioxidant substances were extracted from Camellia oleifera Abel leaves with ethanol. Their antioxidant activities were determined and compared with rutin and BHT by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. EC50 values of DPPH radical scavenging were determined under wavelength of 517nm and reaction time of 40min. The results showed that ethanol extracts from C. oleifera leaves have significant effect on DPPH radical scavenging and the DPPH radical-scavenging efficiency increases with the purification degree of extracts from C. oleifera leaves. EC50 values of the crude extracts, purified extracts from C. oleifera leaves, rutin and BHT are 12.19, 6.765, 9.481 and 37.53mg/L, respectively. The sequence of radical scavenging ability is: purified extracts＞rutin＞crude extracts>>BHT.