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酶法提取黑加仑果渣花色苷的研究

Study on Enzymatic Extraction of Anthocyanins from Blackcurrant Marc

  • 摘要: 通过单因素和正交试验,确定了黑加仑果渣花色苷酶法提取的最佳工艺条件为:釆用纤维素酶酶解,温度50℃,时间100min,果渣与酶解液的比1:9(g:mL),酶用量为原料质量的1%,pH值5.0,黑加仑花色苷提取率8.3%.釆用果胶酶酶解,温度50℃,时间120min,果渣与酶解液的比为1:8(g:mL),酶用量为原料质量的1.4%,pH值3.5,黑加仑花色苷提取率91.09%.并确定了使用果胶酶的提取效果好于纤维素酶.

     

    Abstract: Through experiments, the optimum conditions of enzymatic extraction of anthocyanin from blackcurrant(Ribes nigrum L.) marc, using cellulase were as follows: temperature 50℃,time 100min, ratio of marc to solvent 1:9, amount of enzyme 1% of material, pH value of enzyme solution 5.0, the extraction rate of anthocyanin was 86.63%. Using pectinase, the optimum conditions were: temperature 50℃,time 120min, ratio of marc to solvent 1:8, amount of enzyme 1.4% of material, pH value of enzyme solution 3.5, the extraction rate of anthocyanin was 91.09%. The extraction effect of pectinase is better than that of cellulase.

     

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