Abstract: Seven compounds, including three flavan-3-ols, (+)-catechin (1), (-)-epicatechin (2) and (+)-gallocatechin (3), three flavonoid glycosides, isorhamnetin-3-O-β-D-glucopyranoside (4), astragalin (5) and 2″-O-rhmnosylvitexin (6), and one lignan with the trivial name cedrusin (7), were isolated for the first time from the fresh needles of Larix kaempferi C. Their structures were elucidated by chemical and spectral analysis. The antioxidant activities of the crude extracts, partitioned fractions and isolated compounds were evaluated by DPPH free radical-scavenging assay. Results suggested that EtOAc participated fraction and the three flavan-3-ols showed significantly high antioxidant potential compared with α-tocopherol and BHT, which were used as controls.