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皂荚多糖胶α-半乳糖苷酶修饰及产物性能研究

Studies on Modification of Polysaccharide Gum from Gleditsia sinensis Lam. Seeds with α-Galactosidase

  • 摘要: 皂荚多糖胶(GPG)和刺槐豆胶都属于半乳甘露聚糖型多糖胶.当多糖水溶液质量分数1%,皂荚多糖胶(半乳糖质量分数28.6%)与黄原胶的共混质量比为7:3,其凝胶强度为103g/cm2.刺槐豆胶(半乳糖质量分数20%)与黄原胶的共混凝胶强度最大,也是价格最高的半乳甘露聚糖胶.重点研究了α-半乳糖苷酶修饰皂荚多糖胶(EMGPG)得到与刺槐豆胶相似结构的半乳甘露聚糖,酶转化反应的最适宜底物质量分数从37℃的3%提高到50℃时的35%.20.2%半乳糖含量的酶法改性皂荚多糖胶与黄原胶共混后的凝胶强度为199 g/cm2,大于刺槐豆胶与黄原胶的共混凝胶强度(193g/cm2).X射线衍射分析表明,酶法改性皂荚多糖胶结晶度下降.

     

    Abstract: Gleditsia sinensis Lam. polysaccharides gum (GPG) and locust bean gum were galactomannan gum. Mixed gel at 1% of total polysaccharide concentration showed the gel strength maximum of 103 g/cm2 when the ratio of GPG (28.6% galactose) to xanthan was 7:3. Locust bean gum (20% galactose) is more functional and more expensive than other galactomannan gums. Investigation on removal of galactose residues from GPG by α-galactosidase to yield galactomannan similar to locust bean gum was carried out. The optimum mass fraction of substrate concentrations was increased from 3% at 37℃ to 35% at 50℃ due to increased enzymic temperature tolerance. Enzymically modified G. sinensis polysaccharides gum (EMGPG) with 20.2% galactose content showed maximum mixed gel strength of 199 g/cm2, which was higher than that of locust bean gum (193 g/cm2). The crystallinity index for EMGPG is 0.235, which is somewhat lower than that of GPG (0.297).

     

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