橄榄多酚的提取研究
Study on Extraction of Polyphenols from Canarium album (Lour.) Rauesch.
-
摘要: 利用溶剂浸提法、超声波辅助提取法和微波辅助提取法对橄榄中的多酚物质进行提取研究,并比较了3种方法的提取效果.结果表明:以1g橄榄果肉粉为原料,60%~70%丙酮水溶液是提取橄榄多酚的最适溶剂,最佳提取时间1h,温度20~30℃,固液比1:20(g:mL);超声波提取的最适功率为80W,最佳提取时间25min;微波提取的最适功率为280W,提取时间5s.3种提取方法的多酚提取得率分别为17.1%、19.3%和20.3%,与溶剂浸提法相比,超声波法和微波法提取时间短、效率高,提取效果明显优于前者,并且三者提取物的UV和HPLC检测图谱完全一致.Abstract: Solvent extraction, ultrasonic-assisted extraction and microwave-assisted extraction of polyphenols from Canarium album (Lour.) Rauesch. were studied, and extraction effects of three methods were also compared. The results indicated that 60%-70% aqueous acetone was the preferred solvent for polyphenol extraction, the optimum condition of solvent extraction was as follows: leaching time 1 h, temperature 20-30℃, ratio of material to solvent 1:20, the optimal power and action time for ultrasonic extraction were 80 W and 25 min, while that for microwave extraction were 280 W and 5 s. The polyphenol yields of three methods were 17.1%, 19.3% and 20.3% respectively. As compared with solvent extraction method, ultrasonic-assisted and microwave-assisted methods were more rapid and efficient for extracting polyphenols from C. album, and their UV spectrograms and HPLC chromatograms of polyphenol extracts were identical.
下载: