Abstract: With the adoption of self-designed ultrasonically enhanced supercritical CO2 reversed microemulsion(USCRM) extraction device, and bis(2-ethylhexyl) sodium sulphosuccinate(AOT) with ethanol as main surfactant and co-surfactant respectively, the extraction of ginsenosides with USCRM was studied, and the effects of ultrasound power and operations were observed. According to the experimental results,1 g ginseng was soaked 12 h with 0.6 mL water before extraction, extraction pressure 24 MPa, extraction temperature 45℃, extraction time 4 h, separation pressure 5 MPa, separation temperature 55℃, CO2 flow rate 2.0 L/h.The ginsenosides extraction yield with USCRM is 5.28 and 1.64 times of those with ethanol/supercritical CO2 extraction and with AOT/ethanol/ supercritical CO2 reversed microemulsion extraction respectively.The ginsenosides yield is increased with the increases of ultrasound power and ultrasound extraction time.