Abstract: Physicochemical properties and extraction conditions of the pigment of seeds of Euonymis japonicus Thunb.were investigated. Results showed that good yield of pigment could obtained by acetone extraction, with fresh material:solvent=1:10(g:mL) at 35℃ for 40 min. The pigment is stable against heat, acid-alkali and reducing agent. Under sunlight, degradation of the pigment could be accelerated. Metallic ions,common salt and sucrose had no effect on the tone of pigment. This kind of pigment possesses poor anti-oxidation ability. It is classified to be carotenoids.