Abstract: The extraction of pigment from molasses alcohol wastewater and its antioxidant activity were studied. The macroporous resins HPD-600 and D301-R having better effect were selected through adsorption experiments using 5 macroporous resins on the pigment. The antioxidant activity from pigment from alcohol wastewater was studied in three different systems (NO2-, O2- and DPPH·). The results showed that their antioxidant activities were better in the NO2- and DPPH· system than in the O2- system and their antioxidant activities were enhanced with the increasing of concentration. In nitrite system, the half-inhibition concentraction (IC50) of the pigment toward nitrite was about 0.75 g/L. In DPPH·system, the IC50 of the pigment toward DPPH· was about 0.07 g/L.