Abstract: Ultrasonic extraction of pine seed polysaccharides was studied by using single factor experiment and orthogonal method. It was concluded that the optimal condition of extraction were 100 g degreased pine seed powder, 90℃ extraction temperature, 45:1 (mL:g) solvent to solute weight ratio, 35 min extraction duration, 800 W ultrasonic power. Under this condition, the yield of polysaccharides is 9.74%. Compared with direct-heating extraction, ultrasonic extraction can shorten the extracting time, and improve yield of pine seed polysaccharides.