Abstract: The methods of preserving eleostearic acid were studied, adopting content of eleostearic acid as index. Eleostearic acid was esterificated with BF3 in methanol. The content of eleostearic acid was determined by gas chromatography, and the structure is studied by infrared spectroscopy. The tests on material of container, temperature and condition of preservation have been done. The effects of antioxidant, dosage and temperature on preseration of eleostearic acid were studied through orthogonal test. The results showed that the effects of antioxidant, dosage and temperature on content of eleostearic acid are insignificant. The suitable method for preservation of eleostearic acid is using vacuum package and preserved below 25℃.