柱层析法纯化落叶松木材中的阿拉伯半乳聚糖

STUDY ON PURIFYING ARABINOGALACTAN FROM LARIX GMEINI RUPR. BY COLUMN CHROMATOGRAPHY

摘要: 分别采用大孔吸附树脂柱层析和聚酰胺柱层析技术纯化兴安落叶松中的阿拉伯半乳聚糖,研究了两种层析柱洗脱过程中的杂质含量、糖含量随时间变化的情况,对比分析了它们对阿拉伯半乳聚糖的纯化效果.结果表明,经大孔树脂层析柱纯化后的阿拉伯半乳聚糖产品得率为68.28%,糖含量为95.02%;经聚酰胺层析柱纯化后的产品得率为75.67%,糖含量为98.30%.综合产品得率、糖含量以及成本等多方面因素,选择直接用聚酰胺层析柱来纯化阿拉伯半乳聚糖较为合适.

Abstract: Macroporous adsorptive resin column and polyamide column were used respectively to purify arabinogalactan (AG) from Larix gmeini Rupr.. The contents of impurity in AG and sugar varying with time were analyzed. The purification effects by the two methods were compared, showing that the yield of arabinogalactan purified by the resin was 68.28%, and the sugar content was 95.02%, while those by polyamide column were 75.67% and 98.30%,respectively. The method of polyamide column chromatography is preferred for purifying AG, based on the comparisons of yield, purity and cost.