Abstract: Studies on extraction of extractive and measurements on bacteriostatic activity of extractives against fungi from Melia azedarach L.had been carried out.The optimum extraction conditions of extractive from M.azedarach bark were investigated by using orthogonal experiment as follows:60% ethanol as solvent,ratio of material to liquor 1:9,temperature 60℃ extraction for 4 times and 80 min for each time.Yield of toosendanin is 1.08%.Bacteriostatic activi-ties against Aspergillus niger and Trichoderma viride of extractives respectively from M.azedarach bark and fruit of (different) concentrations,as well as obtained from extraction with different solvents are remarkably different.Extractive from bark possesses higher bacteriostatic activities than extractive from fruit,while ethanol extractive from bark (exhibits) the strongest bacteriostatic activity,with minimum inhibition concentration(MIC) of 0.5% against both (fungi),while MIC of methanol extractive from bark is 2%.