Abstract: Different surfactants were used as assistants to extract rutin from Flos sophorae,from which Tween 20 was confirmed as the best surfactant. The optimal technology is obtained as follows: extracting twice at 60℃ for 30 min,each time with a mass ratio of material to solvent, Tween 20 and borax by 100:700:0.75:0.5,adjusting the extracted liquor′s pH value to 2-3 with 15% hydrochloric acid,laying aside for 40 min to deposit completely followed by separating out the precipitate.Rutin product can be obtained at yield 17.5% and purity 92.5% after drying of the precipitate. Compared with traditional alkaline liquor extraction, the extraction assisted with Tween 20 has the advantages of decreased extracting time and depositing time,and increased yield.