Abstract: The latest researches on the physiological activity of bamboo-leaf-flavonoids(EOB-f) on skin were described as follows:1)Security evaluation tests showed that EOB-f had no off-phenomenon such as pungency and allergy on rat’s skin and eye conjunctiva;2)Chemiluminescence and fluorescence analysis showed that EOB-f was of comparative activities with tea polyphenols and the extract of Ginkgo biloba on anti-free radicals,anti-oxidation,and anti-radiation;3)Bacteria inhibition test in vitro approved that EOB-f was capable of inhibiting various strains of infectious bacteria;4)Anti-inflammation tests revealed that EOB-f has obvious inhibiting effect on mice′s auricle swell caused both by croton oil and dimethylbenzene;5)Cell incubation tests in vitro displayed that,within the concentrations of (0.005%) to 0.05%,EOB-f accelerated significantly the proliferation of two kinds of rat skin cells,kerationcytes and fibroblasts,inhibited the pigment synthesis of B16 black pigment cancer cell,and depressed(MDA) content and enhanced SOD activity of rat skin kerationcytes.In conclusion,EOB-f possesses the essential and sufficient qualities applicable as a kind of safe,effective and economic plant components for anti-aging factor to protect skin in cosmetic.