Abstract: Experiments of six different methods for extracting flavonoids from leaves of Phyllostachys prominensns(W.Y.Xiong) were carried out, namely by reflux extraction, ultrasonic extraction, percolation extraction, cold-soaked extraction, microwave extraction and Soxhlet extraction, adopting flavonoids content as index. The results showed that the flavonoids yields in extracts are in the sequence of: ultrasonic extraction>microwave extraction>reflux extraction>percolation extraction>Soxhlet extraction>cold-soaked extraction. The ultrasonic extraction method was superior to other methods. This method had the advantages of fast extraction speed,no heating, be economical of time, solvent and energy, and had higher yield of flavonoids.