板栗苞单宁纯化的研究

Study on Purification of Chestnut Shell Tannin

摘要: 以通常废弃的板栗苞为原料,经过提取得到板栗苞单宁提取物,并对提取物的纯化进行了研究。分别研究了吸附剂的选择、柱前液质量浓度、洗脱剂、蒸馏水的pH值、流速等单因素对单宁得率的影响,并通过正交试验得出了纯化的最佳工艺条件,即:选用大孔树脂D301为吸附剂,丙酮为洗脱液,柱前液质量分数为 9 %,蒸馏水pH值为7,过柱流速为 0.4 L/h。最后,通过稳定性实验对所得工艺条件进行了验证,按此工艺条件纯化后的板栗苞单宁得率在 82 % 以上,单宁质量分数在 62 % 以上。

Abstract: Purification of chestnut shell tannin from generally abandoned chestnut shell was studied. The effect of adsorbent, pre-column concentration, eluent, pH value of distilled water and flow rate on tannin yield was tested respectively. According to single factor and orthogonal experiment results, the optium experimental conditions were as follows: macroporous resin D301 as adsorbent, acetone as eluent, pre-column concentration 9%, pH value 7.0 of distilled water, flow rate 0.4 L/h. Finally, the best process condition was verified by stability test.Under these conditions, the yield of chestnut shell tannin was up to 82%, and tannin mass fraction was higher than 62%.