Abstract: A polysaccharide was prepared from the branches and leaves of the Taxus chinensis var.mairei by the processing of hot water extraction, concentration, alcohol-precipitation, dialysis and protein isolation successively. The polysaccharide molecular weight was determined by Sephadex G-100 gel permeation chromatography. The monosaccharide composition of the taxus polysaccharide was determined by gas chromatography. And IR spectra of taxus polysaccharide were determined, too. The results showed that the taxus polysaccharide’s molecular weight was 59.2 ku, which composed of 365 monosaccharide groups. The ratio of Rhamnose, Arabinose, Mannose, Glucose and Galactose was 4:6:1:1:4. The taxus polysaccharide was a glycoconjugate that composed of several kinds of monosaccharide, glucuronic acid and amino acid. The monosaccharide was in the form of pyran ring and linked by β-(1,3) glucosidic bond each other. The peptide in taxus polysaccharide couldn't be destructed by Sevag method.