Abstract: An analytic method with Fourier transform infrared spectroscopy (FT-IR) with two dimensional correlation spectroscopy (2D-IR) was established to analyze and evaluate chemical constituent in the different parts of Z.jujuba.var.spinosa. The shape and intensity of the peaks were distinct in single-dimensional FT-IR and the secondary derivative IR spectra of the different parts from Z.jujuba.var.spinosa. Some common peaks in the range of 1750-1300 cm-1 and major peaks were analyzed and assigned to chemical constituent or group. The fingerprint characters of the different parts in 2D-IR spectra were visualized. In the range of 1300-850 cm-1, 3 strong autopeaks arised in the root and leaf, 2 autopeaks in the hardcore,and 1 autopeak in the seed. FTIR combined with 2D-IR was a rapid and comprehensive approach to analyze and evaluate the imperceptible differences among diffe-rent parts of the medicinal herbs.