亚硫酸盐蔗渣浆酶解工艺及外源物辅助作用的研究
The Study about Enzymatic Hydrolysis of Bagasse Sulfite Pulp and the Assistant Effect of Additives
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摘要: 以亚硫酸盐蔗渣浆为研究对象,研究了底物浓度、外源添加物种类和浓度对纤维素酶解工艺的影响以及PEG6000强化酶解效率的作用机理。研究结果表明,纤维素酶用量 15 FPIU/g(以绝干纤维素计,下同)、 β-葡萄糖苷酶用量 30 CBU/g,纤维素质量浓度 80 g/L 条件下水解 48 h,葡萄糖质量浓度达 72.51 g/L,葡萄糖得率、纤维素酶解得率和总糖得率达 81.58%、 86.79% 和 84.23%。PEG6000可有效强化酶解,添加量为 2 g/L 时,水解 48 h 葡萄糖质量浓度可升至 78.54 g/L,葡萄糖得率、纤维素酶解得率和总糖得率达 88.36%、 95.02% 和 92.54%。添加 2 g/L 的PEG6000使纤维素酶Celluclast 1.5 L 滤纸酶活力提高到原酶活力的 117.33%;同时 50℃,pH值4.8,保温 48 h,残余酶活力同比增加 38.99%。Abstract: In order to optimize the hydrolysis conditions of bagasse sulfite pulp, the influences of substrate concentration, species and concentration of exogenous additives on hydrolysis were investigated in present study. The results showed that the sample with 72.51 g/L glucose concentration, 81.58% glucose yield, 86.79% hydrolysis yield and 84.23% total sugar yield were obtained by using 15 FPIU/g cellulase and 30 CBU/g β-glucosidase with 80 g/L cellulose after 48h hydrolysis. Addition of PEG6000 could enhance the hydrolysis process remarkably. The glucose concentration reached 78.54 g/L after 48 h of hydrolysis in presence of 2 g/L PEG6000. Meanwhile, glucose yield, hydrolysis yield and total sugar yield increased to 88.36%, 95.02% and 92.54%, respectively. In addition, 117.33% increase in enzyme activity and 38.99% in stability of Celluclast 1.5L were observed when 2g/L PEG6000 was applied.
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