Abstract: Ultrasound-assisted enzymatic hydrolysis of furfural residues after alkaline peroxide pretreatment was studied. The glucose yields and remaining enzyme activity after enzymatic hydrolysis in different modes were analyzed and compared. The results showed that ultrasound-assisted enzymatic hydrolysis after 2 h of saccharification was the optimized condition and glucose yield in this procedure was enhanced from 55.01% to 73.35% in comparison with traditional procedure. The mechanical effect of ultrasound-assisted affected enzyme adsorption and desorption and was beneficial to enzyme combination with the substrate. The diffe-rent ultrasonic assisted enzymatic hydrolysis were believed to affect the glucose yield and the U-C mode was better than other modes. The yields of enzyme addition after ultrasonic treatment were 46.35% higher than those of enzyme addition before ultrasonic treatment. It is clear that ultrasound-assisted enzymatic hydrolysis of furfural residues after alkaline peroxide pretreatment with low energy consumption and low pollution will provide a good development direction for the industrial production of ethanol.