Abstract: Chemical constituents in the roots of Ziziphus jujuba Mill var.spinosa were studied. The consti-tuents were separated and purified by repeated column chromatagraphy with silica gel and Sephadex LH-20. Their structures were elucidated based on the chemical and spectral analysis. Seven compounds were iso-lated from petroleum,EtOA fractions. Their structures were identified as lupeol(Ⅰ), betulinic acid(Ⅱ), ceanothic acid(Ⅲ), isoceanothic acid(Ⅳ), stigmasterol(Ⅴ), 3-O-β-D-glucopyranosyl-stigmasterol(Ⅵ), adouetine X(Ⅶ). Compounds Ⅳ, Ⅵ, Ⅶ were obtained from the plant for the first time.