Abstract: Effects of polyprenols from Ginkgo biloba L. leaves with different concentrations on wheat seed germination and seedlings growth were studied. The results indicated that the low concentrations (20 and 50 mg/L) of polyprenols could enhance seed germination and seedling growth due to promotion of the activity of amylase and root. Its germination rate was 77.67%, the height was 14.36 cm, the length was 16.4 cm. The activity of amylase and root were 14.75 mg/(g·min) and 109.35 μg/(g·h), respectively. The high concentration (100 mg/L) of polyprenols had inhibition of seed germination and seedling growth. It reduced amylase activity and root activity. The activity of amylase and root were 9.82 mg/(g·min) and 21.12 μg/(g·h), respectively.