水酶法提取辣木油的工艺研究

Aqueous Enzymatic Extraction of Moringa oleifera Lam.Seed Oil

摘要: 通过中性蛋白酶水相溶剂中提取辣木油的单因素和正交试验,研究了影响辣木油水酶法提取的主要因素,得出了水酶法提取辣木油的最佳工艺条件。研究结果表明,最佳条件为:酶用量为辣木籽质量的3.0%,液料比9:1(mL:g),温度50℃,孵育时间18h,pH值5.0的条件下,辣木油平均得率超过28.6%。

Abstract: Through single factors and orthogonal experiments, the optimal condition for aqueous enzymatic extraction of Moringa oleifera Lam.seed oil with neutral protease was studied. The optimal condition is as follows: amount of neutral protease 3% of M.oleifera seed, ratio of enzyme solution to M.oleifera seed 9:1, incubated at 50℃ at pH value 5 for 18h, average oil yield exceeded 28.25%.