Abstract: The optimum technological conditions of extractinghuperzin A from Huperzia serrata(Thunb)Trev. by single factor test and orthogonal design method were studied. The optimum extraction conditions were as follows:ratio of ethanol to sample 25:1(mL:g), extraction time 3h, extraction temperature 60℃. The optimum purification conditions were as follows:ethanol volume fraction 80%, D101 macroporous adsorption resin eluted under room temperature, to obtain an overall yield of 0.3% and a purity of 10%.