Abstract: Voltammetric behavior of rutin at activatedglassy carbon electrode(GCE) was studied .The voltammegram shows a pair of redox peaks when potential scans in a phosphate buffer(PBS) containing rutin. In the phosphate solution of pH value 4.0, the peak current of differential pulse voltammetry (DPV) for rutin rises as its concentration increases, andgood linearity was observed over a range of 0.8-50μmol/L with a detection limit of 0.3μmol/L. This method was developed for the determination of rutin content in plant extracts which is simple and sensitive withgood repeatability. A sample of S.jambos leave was determined with this method in standard-addition way to obtain the rutin content of 15.7mg/g with RSD of 3.3%.