引种阿斯油橄榄叶中橄榄苦苷提取分离及结构鉴定

Isolation and Structrual Identification of Oleuropein in Leaves of Ascolano Olive Introduced from Greece

摘要: 油橄榄叶主要含有橄榄苦苷、黄酮和多酚等抗氧化物。本研究以引种的希腊油橄榄阿斯为原料,采用乙醇-水(体积比60:40)对油橄榄叶进行提取,依次用石油醚和乙酸乙酯萃取,乙酸乙酯萃取相经过硅胶柱层析,再用HPLC制备色谱,得质量分数 95% 以上的单体,通过IR、UV、MS、NMR鉴定该化合物为橄榄苦苷。橄榄苦苷经过酸水解,得到两个主要产物,一个为羟基酪醇,另一个为榄香酸。

Abstract: There are many antioxidants in olive leaves such as oleuropein, flavonoids and polyphenols. Leaves of Ascolano olive introduced from Greece were successively extracted with EtOH-H2O(60:40), petroleum ether and ethyl acetate. The ethyl acetate fraction was subjected to silicagel column chromatography, then treated with preparative HPLC to obtain a compound with content of 95%, which was identificated to be oleuropein by UV,IR,MS and NMR. Oleuropein could be acidicallyhydrolyzed togivehydroxytyrosol and elenolic acid as main products.