Abstract: Perilla oil was extracted with supercritical CO2 extraction, and the supercritical conditions for integrated inclusion of perilla oil was researched using β-cyclodextrin microcapsulation technology. The results show that the optimum conditions were: supercritical extraction pressure of perilla oil 23MPa, the optimum β-cyclodextrin inclusion supercritical pressure 12MPa, inclusion reaction time 180min, inclusion reaction temperature 55℃, extractant CO2 flow rate 40L/min. α-Lindenic acid inclusion rate is 16.37%, the maximum ratio of mass fraction of β-cyclodextrin/perilla oil is 16.42%, the highest molar complexation ratio (β-cyclodextrin/perilla oil) amounts to 1.84:1 (molar ratio of material), average mass fraction of perilla oil/β-cyclodextrin is 13.94%, and the average complexation ratio is 1.55:1(molar ratio of material).